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HEK 293 Cultutre Serum-free Medium for Suspesion Cell

HEK 293 Suspesion Cultutre Serum-free Media

Product use

This medium is used for continuous culture of suspended HEK293 cells (such as 293T, 293S, 293E, 293F) and transient expression of foreign genes, especially in the process of packaging lentivirus.
Product video

HEK293 cells (short for Human Embryonic Kidney 293 cells) are a widely used and versatile tool in the field of biology and the burgeoning cell therapy industry. These cells were originally derived from human embryonic kidney tissue in 1973 and have since become invaluable in various research applications. HEK293 cells exhibit several distinctive characteristics that make them particularly appealing to scientists and biotechnologists.

One of the most notable features of HEK293 cells is their robust ability to grow and replicate in culture, making them highly amenable to genetic manipulation and transfection. This characteristic enables researchers to introduce and study specific genes or proteins, making HEK293 cells indispensable in molecular biology and gene expression studies.

Furthermore, these cells have played a pivotal role in the development of recombinant protein production systems and biopharmaceutical manufacturing. Their high protein expression capability and scalability have positioned them as a cornerstone in the production of various biologics, including vaccines and therapeutic proteins.

In the context of the cell therapy industry, HEK293 cells are instrumental in assessing the safety and efficacy of emerging cell-based therapies. They serve as an essential tool for testing viral vectors, evaluating gene-editing techniques, and studying cellular behavior to ensure the successful development of innovative treatments.

In summary, HEK293 cells have become a foundational resource in the realms of molecular biology, biopharmaceutical production, and cell therapy development. Their versatility, adaptability, and reliability make them a valuable asset in advancing our understanding of biology and the quest for novel therapeutic interventions.


This product is animal serum-free, contains a small number of protein components and it’s chemically defined. This product does not contain glutamine. It is recommended to add it as needed during the cultivation process (the concentration is 2 mM).


Supported cell densities:

4-7x10 6 cells/mL

Transfection efficiency:

50-90%

Packaging lentivirus titers:

>1.5x10 7 TU/mL

Appearance:

light yellow clear liquid

Volume:

1000 mL

Endotoxin

<5 EU/mL

Osmotic pressure

270-310 mOsm/kg

pH

25℃ 6.9-7.4

Shelf life

12 months

Storage conditions:

2-8°C, protected from light


Product advantages

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